An introduction to Somatic gene therapy
Somatic cell gene therapy changes/fixes/replaces genes in a person. The targeted cells are the only ones affected, the changes are not passed on to that person's offspring.
Somatic Gene Therapy can be used to treat many genetic diseases, such as ones in the cultured cells (e.g bone marrow), genetic lung diseases, genetic muscle diseases and genetic liver diseases.
The graph above shows where Gene Therapy is most commonly used:...
Somatic Gene Therapy can be used to treat many genetic diseases, such as ones in the cultured cells (e.g bone marrow), genetic lung diseases, genetic muscle diseases and genetic liver diseases.
The graph above shows where Gene Therapy is most commonly used:...
Scientific prinipciples of sOmatic gene therapy
SCIENTIFIC PRINCIPLE ONE: GENETIC CLONING
Gene cloning is the process in which a gene of interest is located and copied (cloned) out of DNA extracted from an organism. When DNA is extracted from an organism, all of its genes are extracted at one time. This DNA contains thousands of different genes. The genetic engineer must find the one specific gene that encodes the specific protein of interest.
Since there is no way to locate a gene by visibly looking at all of the DNA, scientists make gene libraries to catalogue the organism's DNA. The gene the scientist is looking for is selected from this library.
It can be used for many different purposes, such as creating GM crops, finding a cure for disease or for gene therapy.There are two types of gene cloning: 'in vivo', which involves the use of restriction enzymes and ligases using vectors and cloning the fragments into host cells. The other type is 'in vitro' which is using the polymerase chain reaction (PCR) method to create copies of fragments of DNA...
Gene cloning is the process in which a gene of interest is located and copied (cloned) out of DNA extracted from an organism. When DNA is extracted from an organism, all of its genes are extracted at one time. This DNA contains thousands of different genes. The genetic engineer must find the one specific gene that encodes the specific protein of interest.
Since there is no way to locate a gene by visibly looking at all of the DNA, scientists make gene libraries to catalogue the organism's DNA. The gene the scientist is looking for is selected from this library.
It can be used for many different purposes, such as creating GM crops, finding a cure for disease or for gene therapy.There are two types of gene cloning: 'in vivo', which involves the use of restriction enzymes and ligases using vectors and cloning the fragments into host cells. The other type is 'in vitro' which is using the polymerase chain reaction (PCR) method to create copies of fragments of DNA...
SCIENTIFIC PRINCIPLE TWO: PROCESS AND VIRAL VECTORS USED IN SOMATIC GENE THERAPY
The process of Somatic Gene Therapy entails inserting normal genes into the cell/s of an individual with the genetic disease and, thus, eradicating the disease completely.
The diagram below outlines the simplest methods of getting genes into the person's cells using either viruses (which carry the human gene, in place of one of their own genes, into a cell) or liposomes (small molecules which can carry DNA into a cell).
In some cells, the gene/s are inserted into a chromosome in the nucleus...
The process of Somatic Gene Therapy entails inserting normal genes into the cell/s of an individual with the genetic disease and, thus, eradicating the disease completely.
The diagram below outlines the simplest methods of getting genes into the person's cells using either viruses (which carry the human gene, in place of one of their own genes, into a cell) or liposomes (small molecules which can carry DNA into a cell).
In some cells, the gene/s are inserted into a chromosome in the nucleus...
kareem HABIB